Journal of Biochemical & Biophysical Methods. 22(1):69-82, 1991 Jan.

A new method for the determination of the permittivity of small samples in the microwave range and its application to hemoglobin single crystals.

Daseler W, Steinhoff HJ, Redhardt A

Institut fur Biophysik, Ruhr-Universitat Bochum, F.R.G.

A cavity perturbation method for the absolute determination of the complex permittivity of small samples in the microwave range is developed and tested. Samples with volumes less than 0.4 mm3, for example protein powder or single crystals of macromolecules, may be investigated in a temperature range between 180 and 300 K, using this method. As an application the complex permittivity of hemoglobin single crystals is determined at three frequencies, v = 3.06 GHz, v = 8.76 GHz and v = 17.0 GHz in a temperature range between 180 and 300 K.