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Photosynthetic reaction center


Photosynthetic reaction center (RC) of Rhodobacter sphaeroides, due to the availability of its crystal structure, accessibility of site specific mutants and possibility to trigger the single enzyme turnovers by short flashes of light, is a paradigm study object in biophysics. We have tried to understand how ubiquinone is reduced and protonated to ubiquinol in this enzyme. By combining spectroscopic and electrometric measurements with kinetic modeling and molecular dynamics simulations we obtained a mechanistic scheme where the relocations of the ubiquinone ring in its binding pocket were kinetically related to the two electron and two proton transfer steps.

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Figure taken from [3]

 

 

 

Reduction of the secundary quinone in the Reaction centre of Rhodobacter sphaeroides.
© D. Cherepanov, A. Mulkidjanian, 2000


References

1. Kozlova, M.A., H.D. Juhnke, D.A. Cherepanov, C.R.D Lancaster, A.Y. Mulkidjanian. 2008. Proton transfer in the photosynthetic reaction center of Blastochloris viridis, FEBS Letters, 582, 238-242.(Full text, pdf)

2. Mulkidjanian,A.Y., D.A.Cherepanov, and M.A.Kozlova. 2005. Ubiquinone reduction in the photosynthetic reaction center of Rhodobacter sphaeroides: Interplay between electron transfer, proton binding and the flips of quinone ring. Biochem. Soc. Trans. 33:845-850. (Full text, pdf)

3. Kozlova,M.A., D.A.Cherepanov, L.Baciou, P.Sebban, and A.Y.Mulkidjanian. 2005. Electron transfer coupled protein relaxation in reaction centers of Rb. sphaeroides. In Photosynthesis: Fundamental aspects to global perspectives. A.van der Est and D.Bruce, editors. Montreal. 222-224.

4. Junge, W., M. Haumann, R. Ahlbrink, A. Mulkidjanian, and J. Clausen. 2002. Electrostatics and proton transfer in photosynthetic water oxidation. Philos. Trans. R. Soc. Lond B Biol Sci. 357:1407-1417.(Full text, pdf)

5. Ahlbrink, R., Semin, B.K., Mulkidjanian, A.Y & Junge, W. 2001. Photosystem II of peas: Effects of added divalent cations of Mn, Fe, Mg, and Ca on two kinetic components of P680+-reduction in Mn-depleted core particles, Biochim. Biophys. Acta, 1506, 117-26.(Full text, pdf)

6. Cherepanov, D.A., Krishtalik. L.I., Mulkidjanian, A.Y., 2001. Photosynthetic electron transfer controlled by protein relaxation: Analysis by Langevin stochastic approach, Biophysical Journal, 80, 1033-1049.(Full text, pdf)

7. Klishin,S.S., D.A.Cherepanov, and A.Y.Mulkidjanian. 2001. Resolution of proton and electron transfer events in the photosynthetic reaction center and the cytochrome-bc1 complex of phototrophic bacteria. In PS2001. 12th International Congress of Photosynthesis. Brisbane. S12-005 (electronic publication).(Full text, pdf)

8. Cherepanov, D.A., S.I. Bibikov, M.V. Bibikova, D.A. Bloch, L.A. Drachev, O.A. Gopta, D. Oesterhelt, A.Y. Semenov, and A.Y. Mulkidjanian. 2000. Reduction and protonation of the bound QB ubiquinone in the photosynthetic reaction centers of Rb. sphaeroides: Kinetic model based on a comparison between wild type chromatophores and the mutants with arginine substituted by isoleucine at 207 and 217 sites in the L-subunit. Biochim.Biophys.Acta, 1459, 10-34.(Full text, pdf)

9. Mulkidjanian, A.Y. 1999. Conformationally controlled pK-switching in membrane proteins: One more mechanism specific to the enzymatic catalysis? FEBS Lett., 463, 199-204.(Full text, pdf)

10. Gopta, O.A., D.A. Cherepanov, W. Junge, and A.Y. Mulkidjanian. 1999. Proton transfer from the bulk to the bound ubiquinone QB of the reaction center in chromatophores of Rhodobacter sphaeroides: Retarded conveyance by neutral water. Proc.Natl.Acad.Sci.U.S.A. 96:13159-13164.(Full text, pdf)

11. Mulkidjanian, A.Y. 1999. Photosystem II of green plants: On the role of retarded protonic relaxation in water oxidation, Biochim. Biophys. Acta, 1419, 1-6.(Full text, pdf)

12. Haumann, M., A.Y. Mulkidjanian, W. Junge. 1999. Tyrosine-Z in oxygen-evolving photosystem II: A hydrogen-bonded tyrosinate? Biochemistry, 38, 1258-1267.(Full text, pdf)

13. Mulkidjanian, A.Y., A. Hochkoeppler, D. Zannoni, L.A. Drachev, B.A. Melandri and G. Venturoli. 1998. Photosynthetic electrogenic events in native membranes of Chloroflexus aurantiacus. Flash-induced charge displacements in the acceptor side of the photosynthetic reaction center, Photosynth. Res. 56, 75-82. (Full text, pdf)

14. Gopta, O.A., D.A. Cherepanov, A.Y. Semenov, A.Y. Mulkidjanian and D.A. Bloch. 1998. Effect of temperature and surface potential on the electrogenic proton uptake in the QB site of the Rhodobacter sphaeroides reaction center: QA?•QB?• ? QAQBH2 transition, Photosynth. Res. 55, 309-316. (Full text, pdf)

15. Ahlbrink, R., M. Haumann, M., D.A. Cherepanov, O. Bögershausen, A.Y. Mulkidjanian., and W. Junge. 1998. The function of tyrosine-Z in photosystem II of green plants: Effects of the pH, the temperature, and the H/D-isotopic ratio on the rates and extents of electron transfer, proton release and local electrochromism in the inactive and oxygen-evolving preparations, Biochemistry, 37, 1131-1142.(Full text, pdf)

16. Cherepanov, D.A. and A.Y. Mulkidjanian. 1998. Effect of dielectric relaxation on the kinetics of electron transfer in photosynthetic reaction centers. In Photosynthesis: Mechanisms and Effects. G. Garab, editor. Kluwer Academic Publishers, Dordrecht. 795-798.

17. Cherepanov, D.A., W. Drevenstedt, L.I. Krishtalik, A.Y. Mulkidjanian, and W. Junge. 1998. Protein relaxation and kinetics of P680+ reduction in photosystem II. In Photosynthesis: Mechanisms and Effects. G. Garab, editor. Kluwer Academic Publishers, Dordrecht. 1073-1076.

18. Gopta, O.A., D.A. Cherepanov, W. Junge, and A.Y. Mulkidjanian. 1998. Proton transfer from the bulk to the secondary quinone acceptor in chromatophores of Rhodobacter sphaeroides. In Photosynthesis: Mechanisms and Effects. G. Garab, editor. Kluwer Academic Publishers, Dordrecht. 869-872.

19. Gopta O.A., D.A. Bloch, D.A. Cherepanov and A.Y. Mulkidjanian. 1997. The temperature dependence of the electrogenic reaction in the QB site of the Rhodobacter sphaeroides photosynthetic reaction center: the QA?QB ? QAQB? transition, FEBS Letters,412, 490-494. (Full text, pdf)

20. Haumann, M., A.Y. Mulkidjanian, W. Junge. 1997. The electrogenicities of electron and proton transfer at the oxidizing side of photosystem II, Biochemistry, 36, 9304-9315.(Full text, pdf)

21. Mulkidjanian, A.Y., D.A. Cherepanov, M. Haumann and W. Junge. 1996. Photosystem II of green plants: Topology of core pigments and redox cofactors as inferred from difference electrochromic spectra. Biochemistry, 35:3093-3107.(Full text, pdf)

22. Mulkidjanian, A. and W. Junge. 1996. New photosynthesis or old?. Nature, v. 379, p.304-305(Full text, pdf)

23. Cherepanov, D.A., M. Haumann, W. Junge and A.Mulkidajnian. 1995. Topology of chromophores and cofactors in PSII as derived from the local electrochromic bandshifts. p. 531-534, In: Photosynthesis: From Light to Biosphere, Vol. I., Kluver Academic Publishers, Dordrecht.

24. Mulkidjanian, A. , G. Venturoli, A. Hoechkoepler, D. Zannoni, B. A. Melandri, and L. Drachev. 1994. Photosynthetic electrogenic events in native membranes of Chloroflexus aurantiacus: Flash-induced charge displacements within the reaction center-cytochrome c554 complex. Photosynth. Res. 41:135-143.

25. Shinkarev, V. P., L. A. Drachev, M. D. Mamedov, A. J. Mulkidjanian, A. Y. Semenov, and M. I. Verkhovsky. 1993. Effect of pH and surface potential on the rate of electric potential generation due to proton uptake by secondary quinone acceptor of reaction centers in Rhodobacter sphaeroides chromatophores. Biochim. Biophys. Acta 1144:285-294.(Full text, pdf)

26. Venturoli, G. , A. Mulkidjanian, A. Hochkoepler, M. Trotta, R. Feick, B. A. Melandri and D. Zannoni. 1992. Studies on the quinone acceptor complex in the photosynthetic reaction center of the termophilic bacterium Chloroflexus aurantiacus. p. 161-163. In; Thermophils: Science and Tecnology. Reikjavik.

27. Drachev, L. A., M. D. Mamedov, A. Y. Mulkidjanian, A. Y. Semenov, V. P. Shinkarev, and M. I. Verkhovsky. 1990. Electrogenesis associated with proton transfer in the reaction center protein of purple bacteria Rhodobacter sphaeroides. FEBS Letters 259:324-326.(Full text, pdf)

28. Mulkidjanian, A. Y., M. D. Mamedov, A. Y. Semenov, V. P. Shinkarev, M. I. Verkhovsky, and L. A. Drachev. 1990. Partial reversion of electrogenic reaction in ubiquinol:cytochrome c2 reductase of Rhodobacter sphaeroides under the neutral and alkaline conditions. FEBS Letters 277:127-130.(Full text, pdf)

29. Verkhovskii, M. I., L. A. Drachev, M. D. Mamedov, A. Y. Mulkidjanyan, A. Y. Semenov, and V. P. Shinkarev. 1990. The electrogenic step associated with the secondary acceptor semiquinone formation in the reaction centers of purple bacteria. Biokhimiya 55:387-394.

30. Verkhovsky, M. I., N. P. Grishanova, M. D. Mamedov, A. Y. Mulkidjanian, A. Y. Semenov, and V. P. Shinkarev. 1990. The mechanism of interaction between the photosynthetic reaction center and cytochrome-bc1 complex in chromatophores from the non-sulphur purple bacterium Rhodobacter sphaeroides. Biokhimiya 55:1202-1209.

31. Shinkarev, V. P., A. L. Drachev, M. D. Mamedov, A. Y. Mulkidjanian, A. Y. Semenov and M. I. Verkhovsky. 1990. Light-induced electron transfer and electrogenic reactions in the cytochrome-bc1 complex of photosynthetic purple bacterium Rhodobacter sphaeroides. p. 393-400. In Molecular Biology of Membrane-Bound Complexes in Phototrophic Bacteria (G. Drews and E. A. Dawes, eds.). Plenum Press, New York.

32. Drachev, L. A., M. D. Mamedov, A. Y. Mulkidjanian, A. Y. Semenov, V. P. Shinkarev, M. I. Verkhovsky, B. S. Kaurov, and V. P. Skulachev. 1989. Flash-induced electrogenic events in the photosynthetic reaction center and cytochrome-bc1 complex of Rhodobacter sphaeroides chromatophores. Biochim. Biophys. Acta 973:189-197.(Full text, pdf)

33. Drachev, L. A., M. D. Mamedov, A. Y. Mulkidjanian, A. Y. Semenov, V. P. Shinkarev, and M. I. Verkhovsky. 1989. Transfer of ubiquinol from the reaction center to the cytochrome-bc1 complex in Rhodobacter sphaeroides chromatophores. FEBS Letters 245:43-46.(Full text, pdf)

34. Verkhovsky, M. I., L. A. Drachev, M. D. Mamedov, A. Y. Mulkidjanian, A. Y. Semenov, and V. P. Shinkarev. 1989. Electrogenic protonation of the reaction center ubiquinone in chromatophores of non-sulphur purple bacteria. Dokl. Akad. Nauk SSSR 304:209-212.

35. Drachev, L. A., M. D. Mamedov, A. Y. Mulkidjanian, A. Y. Semenov, V. P. Shinkarev, and M. I. Verkhovsky. 1988. Phase II of carotenoid bandshift is mainly due to the electrogenic protonation of the secondary quinone acceptor. FEBS Letters 233:315-318.(Full text, pdf)

36. Mulkidjanian, A. Y., V. P. Shinkarev, M. I. Verkhovsky, and B. S. Kaurov. 1986. A study of kinetic properties of the stable semiquinone of the reaction center secondary acceptor in chromatophores of non-sulfur purple bacteria. Biochim. Biophys. Acta 849:150-161.(Full text, pdf)

37. Mulkidjanian, A. Y., M. I. Verkhovsky, V. P. Shinkarev, V. D. Sled', N. P. Grishanova, and B. S. Kaurov. 1985. Study of photosynthetic electron transfer reactions by redox probes: Electron transport in non-sulphur purple bacteria. Biokhimiya 50:1786-1796.

38. Shinkarev, V. P., A. Y. Mulkidjanian, M. I. Verkhovsky, and B. S. Kaurov. 1985. Investigation of the kinetic and thermodynamic properties of the photosynthetic reaction center secondary acceptor in chromatophore membrane of nonsulphur purple bacteria. Biol. Membrany 2:725-737.

39. Sled', V. D., A. Y. Mulkidjanian, V. P. Shinkarev, M. I. Verkhovsky, B. S. Kaurov, and A. B. Rubin. 1984. Direct spectrophotometric determination of the midpoint potential of primary and secondary quinone acceptors of photosynthetic reaction center. Biokhimiya 49:204-208.

40. Verkhovsky, M. I., B. S. Kaurov, A. Y. Mulkidjanian, V. D. Sled', D. A. Cherepanov, and V. P. Shinkarev. 1984. Study of photosynthetic electron transfer reactions by redox probes: Principles of dynamic redox potentiometry. Biokhimiya 49:394-402.>

41. Shinkarev, V. P., M. I. Verkhovsky, V. D. Sled', A. Y. Mulkidjanian, A. V. Zherdev, D. A. Cherepanov and B. S. Kaurov. 1984. Light-induced electron transfer, proton consumption and transmembrane potential difference formation in chromatophores of non-sulphur purple bacteria. p. 157-163. In Solar Energy Bioconversion. (I. V. Berezin, ed.), Pushchino.

42. Sled', V. D., M. I. Verkhovsky, V. P. Shinkarev, A. Y. Mulkidjanian, N. P. Grishanova, and B. S. Kaurov. 1983. Interactions of redox-mediators with chromatophores of photosynthetic bacterium Rhodospirillum rubrum. Molek. Biologiya 17:33-41.

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Letzte Änderung: 22.11.2017 Katrin Jahns